Caracterização Genômica e Prospecção In Silico de Metabólitos Secundários da Cianobactéria Nostoc Gbbb01 do Parque Nacional da Chapada das Mesas-MA

Abstract

Cyanobacteria are photosynthetic prokaryotic microorganisms that are known to produce a variety of secondary metabolites with diverse bioactivities. Although the state of Maranhão presents a unique combination of biodiversity since it has three Brazilian biomes (Amazon, Cerrado and Caatinga) and respective transition zones, few studies have been carried out to characterize the diversity of photosynthesizers and their potential. In view of this, this study aimed to characterize the genome of the Nostoc sp. GBBB01 from the collection of the UFMA Biodiversity, Bioprospecting and Biotechnology Research Group, isolated in unialgal culture originally from the Chapada das Mesas National Park, in the Cerrado of Maranhão, and investigating by means of genomic mining secondary metabolites of biotechnological interest. After metagenomic sequencing, the genomes obtained were separated and assembled and then proceeded with the step of annotating the isolated genome of the GBBB01 strain using the Prokaryotic Genome Annotation Pipeline (PGAP) and RAST. Structural and comparative characterization of the genome was performed using antiSMASH 5.0, NaPDoS, OrthoVven2, MEGA X for phylogeny and Species Tree application present in KBase for phylogenomics. Phenetic analysis of the 16S rRNA sequence of the Nostoc sp. GBBB01 confirmed the strong relationship with other strains of the genera Nostoc and Desmonostoc. 25 functional categories were identified by the RAST server. The search performed by the antiSMASH tool detected 24 gene clusters of biosynthetic pathways. When comparing individual results between genomes, the most common groupings were bacteriocins, terpenes, heterocyst glycolipids, NRPS, nostoficin, microviridine k and nostopeptolide A2, but only in the GBBB01 genome did we detect the biosynthetic pathways of nosperine, anabaenopeptin / nostamide A. C and KS domains were identified 15 and 21 respectively. This is the first record of a genome of the genus Nostoc from the state of Maranhão, the strain demonstrated the presence of genes related to several compounds with importance from the point of view of health and environment, in addition to revealing its high potential in producing bioactive compounds relevant to biotechnological applications.