Bioprospecção tecnológica da lipase exógena extraída e purificada de Fusarium solani

Abstract

Introduction: Lipases produced by microorganisms have shown rapid advancement in the field of enzymatic technology. They can act on a variety of substrates. Microbial origin lipases play an interesting role in biotechnology because many of them are stable across a wide range of pH, high temperatures, and organic solvents. Objective: To produce the lipase enzyme from the fungus Fusarium solani for biotechnological applications in the health, aesthetics, and industry sectors. Methods: The fungus Fusarium solani was cultivated on solid media for morphological identification through analysis of its macroscopic and microscopic characteristics via microcultivation, followed by submerged fermentation and semi-solid fermentation. The enzyme was then purified, and the lipolytic activity of the extracts was assessed by testing pH, temperature, surfactant effects, and storage time. Additionally, the cellular viability of the extracts, antioxidant activity, and animal fat degradation were also investigated. Results: Submerged fermentation using olive oil as a substrate resulted in the production of Lipase F2 with notable activity. Lipase F2 exhibited stability at pH 8.0, maintaining a lipolytic activity of 135.89 U/mg with a purity degree of 3.63. It remained active within the temperature range of 35 to 70°C, with optimal stability at 35°C, and demonstrated resistance to various surfactants and ions. Tests with cells showed no significant difference in cellular viability when treated with the lipase enzyme from F. solani. Chicken adipose tissue was degraded from 10g to 0.89g in 96 hours in the presence of the purified F. solani lipase. The characterization and production results of the microemulsion incorporated with Fusarium solani lipase indicate that it is a stable formulation with proven efficacy. Conclusion: Therefore, an emulsion containing the lipase enzyme from Fusarium solani is viable for industrial use, being favorable and safe for human use without toxicity.