MODULAÇÃO DA INFLAMAÇÃO ASSOCIADA AO MODELO ASCÍTICO DE TUMOR DE EHRLICH DA FORMULAÇÃO NANOESTRUTURADA CONTENDO O EXTRATO DAS FOLHAS DE Fridericia Platyphylla

Abstract

Cancer is a multifactorial disease that arises from gene mutations mediated by errors during DNA replication. Traditional treatments available, in addition to presenting many adverse effects and toxicity, are ineffective in treating resistant tumors. Therefore, the search for new therapeutic strategies with low toxicity, greater specificity and greater efficacy is essential. In this scenario, the objective of this study was to evaluate the modulation of inflammation mediated by the nanostructured formulation containing the extract of Fridericia platyphylla leaves in an Ehrlich ascites tumor model. For the experimental assay, 44 Swiss mice were distributed in 6 groups (healthy, negative control, positive control, vehicle, and nanostructured formulation in two doses, 10 and 25 mg/kg) and treated for 8 consecutive days. On days 1, 5 and 8, the weight and abdominal circumference of the animals were recorded. After the last day of treatment, the animals were euthanized for collection of ascitic fluid, lymphoid organs, lining of the inner lining of the peritoneal wall, bone marrow and blood for cell viability assays, cytokine levels, nitric oxide, cell cycle, cell death and immunophenotyping. Regarding Ehrlich ascitic tumor, there was an increase in the total weight of the animals due to the growth of tumor cells. The treatments, particularly at a dose of 25 mg/kg, reduced the parameters associated with tumor development, such as weight, abdominal circumference, volume, cellularity. In addition, they did not demonstrate relevant effects on the type of cell death triggered or changes in the cell cycle of tumor cells. Treatment at the highest concentration was able to increase the frequency of CD11c+ cells, T helper lymphocytes and CD3+CD4+ cells. The increase in the population of dendritic cells in the ascites fluid and the cytokine profile in the tumor microenvironment suggests a more suppressive profile, notably due to the decrease in the cytokines TNF-α, IFN-γ and even IL-10. The formulation of F. platyphylla leaves did not promote direct cytotoxic effects in vivo, but presented an interesting immunomodulatory potential, particularly in the regulation of tumor-associated inflammation.