Efeito do óleo de Copaifera langsdorffi Desf e/ou associação com Arnica montana L. em modelo experimental de tendinopatia

Abstract

Introduction: Tendinopathy causes a reduction in the labor and occupational activities of the person affected by this disease, which generates a high impact on their quality of life. Pharmacological therapy for this type of injury is still restricted to the use of analgesics and anti-inflammatories that have adverse effects and high financial cost. Objective: To evaluate the use of Copaifera langsdorffi Desf. oil and/or association with Arnica montana L. in the inflammation of the Achilles tendon. Method: This is an in vivo experimental study, carried out with adult Rattus norvegicus with the disease induced by intratendinous injection of collagenase. These animals were randomly distributed into groups according to the respective therapeutic treatment (n=5/group): Healthy Group; CTL Group (negative control); MLX Group (Meloxicam); OCPB- Group (Copaifera langsdorffii Desf.); A.M.- Group (Arnica montana L.); OCPB + A.M Group (Copaifera langsdorffii Desf. + Arnica montana L). The evaluations of the used therapy were made through the measurement of the tendon thickness, weight-bearing deficit in the ipsilateral paw, spontaneous ambulation test, cytokine analysis, histopathological aspects of the tissue and analysis of the proportion of collagen types I and III. Results: Copaifera langsdorffi Desf. oil in a single dose or associated with Arnica montana L. showed an improvement in the weight distribution on the affected paw when compared to the negative control, throughout the treatment, reaching the end of the treatment with the average increase in distribution on the affected paw of the treated group A.M 50 mg/kg (52.21%), OCPB 50 mg/kg Group (48.60%) and OCPB + A.M (50.17%). Oral administration of Copaifera langsdorffii Desf. oil (50 mg/kg) in a single daily dose and combined promoted a 95% reduction in the thickness of the Achilles tendon inflammation, and the combined dose reduced it by 92%. In the evaluation of spontaneous ambulation, there was a statistically significant increase in the average speed of animals treated with Copaifera langsdorffii Desf. and the associated dose (34.20%). Cytokine analysis showed a significant reduction in IL-1β in animals treated with the Copaifera langsdorffii Desf. association and the association with Arnica montana L.. Histological analysis showed no difference in the comparison regarding the parameters of inflammation and tendon neoangiogenesis between the groups. However, in the parameters of tendon fiber arrangement and rounding of the fibroblast nucleus, it was observed that in treatments with Copaifera langsdorffii Desf. oil. in a single dose and in combination, showed a reduction in the parameters of tendon fiber arrangement, proving positive regulation in tissue remodeling. In the analysis of the tendon tissue of animals treated with Copaifera langsdorffii Desf., more compact collagen fibers, oxygenated cells and cells rich in collagen were observed. Conclusion: Copaifera langsdorffii Desf. oil in combination with Arnica montana L. presents an improvement in tendon tissue in the parameters of tendon fiber arrangement of hypercellularity, a rounding of the fibroblast nucleus and an increase in the proportion of collagen, thus evidencing an anti-inflammatory effect.