Síntese e caracterização do complexo de cobre(II) com os ligantes 1,10-fenantrolina e prolina para potencial uso como antitumoral

Abstract

Cancer differs in a very specific way from other diseases because there is an abnormal cellular perspective, affecting the processes of other cells, spreading this disorder, and can be restricted to various parts of the body. Because of this, cancer is one of the great challenges facing medicine. In this context, the objective of this work is to obtain a crystal from copper(II), 1,10- Phenanthroline and proline and to carry out the study of aquatic, optical, vibrational and thermal properties of the material, in addition to a computational study and a antitumor activity test of the formed coordination complex. The crystal was obtained after a period of 10 days with dark blue needle-shaped colors, characterized by XRD, with Rietveld refinement, UV-Vis, FT-IR, Raman, TG, DTA, DSC, XRD as a function of temperature with Le Bail refinement, evaluation of in vitro antitumor activity and a theoretical study of the coordination complex was performed using DFT. According to UV-Vis spectroscopy, the complexation of the crystal occurred at pH 6.3, XRD at room temperature showed that the complex has a monoclinic structure with space group P21. The DFT study proved that the electrophilicity index (ω) of the studied coordination complex is higher than that of other compounds reported in the literature, which is an important

parameter in the study of the potential of a material to present antitumor activity. From the FT- IR and Raman, the complexation of the copper(II) ion with 1,10-Phenanthroline and proline

was evidenced. The thermal studies indicated that the material has thermal stability at 52oC, that is, higher than the normal temperature of the human body, which favors its use as an antitumor, in addition to presenting phase transformation from 57oC. XRD analysis as a function of temperature confirmed the events indicated in the thermal analysis and through refinement by the Le Bail method, the crystallographic data of the new phase were obtained, with modification of its system from monoclinic to triclinic of space group P1. The biological tests confirmed the antitumor activity of the coordination complex, for the PC3 and SNB-19 strains the IC50 values remained respectively 1.5 and 2.9 μM. The biological activity presented by the complex studied is greater than that of cisplatin and other copper complexes reported in the literature. Thus, the results obtained in this study suggest that the complex of copper(II) with the ligands 1,10-Phenanthroline and Proline has potential for application in the chemotherapy treatment of cancer cells.